polyclonal antibody against lif  (R&D Systems)

Journal: Comparative Hepatology

Article Title: Expression of leukemia inhibitory factor (LIF) and its receptor gp190 in human liver and in cultured human liver myofibroblasts. Cloning of new isoforms of LIF mRNA

doi: 10.1186/1476-5926-3-10

Figure Lengend Snippet: Detection of LIF transcripts in cultured human liver myofibroblasts. ( A ): Northern blot. Total RNA from cultured human liver myofibroblasts was hybridized with a cDNA probe to human LIF. A single 4.5 kb band was observed; ( B ) and ( C ): RT-PCR. Total RNA was subjected to reverse transcription then to PCR with the hLIF-D3/hLIF-N4 ( B ) or with the hLIF-M3/hLIF-N4 primers ( C ).

Article Snippet: A commercially available polyclonal antibody against human LIF (R&D Systems, Minneapolis, Minnesota, USA), and different monoclonal antibodies against gp190, previously described [ ], were used at concentrations optimised on control tissues.

Techniques: Cell Culture, Northern Blot, Reverse Transcription Polymerase Chain Reaction, Reverse Transcription

Journal: Comparative Hepatology

Article Title: Expression of leukemia inhibitory factor (LIF) and its receptor gp190 in human liver and in cultured human liver myofibroblasts. Cloning of new isoforms of LIF mRNA

doi: 10.1186/1476-5926-3-10

Figure Lengend Snippet: Primers used for PCR

Article Snippet: A commercially available polyclonal antibody against human LIF (R&D Systems, Minneapolis, Minnesota, USA), and different monoclonal antibodies against gp190, previously described [ ], were used at concentrations optimised on control tissues.

Techniques:

Journal: Comparative Hepatology

Article Title: Expression of leukemia inhibitory factor (LIF) and its receptor gp190 in human liver and in cultured human liver myofibroblasts. Cloning of new isoforms of LIF mRNA

doi: 10.1186/1476-5926-3-10

Figure Lengend Snippet: Sequence of LIF-D, M and T isoforms. Exons D, M and T are the 3 alternate first exons. Primers used for PCR are underlined. Primers hLIF-M2, M3 and M5 cover the same sequence but differ because of the presence or the absence of restriction sites.

Article Snippet: A commercially available polyclonal antibody against human LIF (R&D Systems, Minneapolis, Minnesota, USA), and different monoclonal antibodies against gp190, previously described [ ], were used at concentrations optimised on control tissues.

Techniques: Sequencing

Journal: Comparative Hepatology

Article Title: Expression of leukemia inhibitory factor (LIF) and its receptor gp190 in human liver and in cultured human liver myofibroblasts. Cloning of new isoforms of LIF mRNA

doi: 10.1186/1476-5926-3-10

Figure Lengend Snippet: RT-PCR analysis of LIF-M expression in various cell lines and in human liver. ( A ): LIF-M expression was analyzed with the hLIF-M2 and hLIF-3N primers: Line 1, human liver myofibroblasts; Line 2, HepG2; Line 3, Hep3B; Line 4, HuH7; Line 5, HEK293. Product sizes are shown in bp; ( B ): normal human liver samples. LIF-D expression was analyzed with the hLIF-D3 and hLIF-N4 primers in 4 different samples. The same samples also expressed LIF-M (not shown). Product sizes are shown in bp; ( C ): diseased human liver samples. In that case, LIF-M expression was analyzed with the hLIF-M3 and hLIF-4N primers in 4 cases of cirrhotic liver. The same samples also expressed LIF-D (not shown). Product sizes are shown in bp; ( D ): semi-quantitation of LIF-D and s-LIF-D expression in a human liver myofibroblasts sample. LIF-D expression was analyzed with the hLIF-D3 and hLIF-N4 primers. The left part shows the migration pattern of the PCR-amplified products with the number of cycles above and the size of the products indicated by arrows, in bp. The graph on the right shows the signal quantification. Similar results were obtained with LIF-M.

Article Snippet: A commercially available polyclonal antibody against human LIF (R&D Systems, Minneapolis, Minnesota, USA), and different monoclonal antibodies against gp190, previously described [ ], were used at concentrations optimised on control tissues.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Quantitation Assay, Migration, Amplification